| dc.creator |
Casas Cendoya, Ana Maria |
|
| dc.creator |
Contreras‐Moreira, Bruno |
|
| dc.creator |
Cantalapiedra, Carlos P. |
|
| dc.creator |
Sakuma, Shun |
|
| dc.creator |
Gracia Gimeno, Ma. Pilar |
|
| dc.creator |
Moralejo Vidal, Mª Angeles |
|
| dc.creator |
Molina Cano, José Luis |
|
| dc.creator |
Komatsuda, Takao |
|
| dc.creator |
Igartua Arregui, Ernesto |
|
| dc.date |
2018-04-12 |
|
| dc.date.accessioned |
2025-11-03T12:17:38Z |
|
| dc.date.available |
2025-11-03T12:17:38Z |
|
| dc.identifier |
https://doi.org/10.1007/s11032-018-0816-z |
|
| dc.identifier |
1380-3743 |
|
| dc.identifier |
http://hdl.handle.net/10459.1/69847 |
|
| dc.identifier.uri |
http://fima-docencia.ub.edu:8080/xmlui/handle/123456789/24554 |
|
| dc.description |
Six-rowed spike 1 (Vrs1) is a gene of major importance for barley breeding and germplasm management as it is the main gene determining spike row-type (2-rowed vs. 6-rowed). This is a widely used DUS trait, and has been often associated to phenotypic traits beyond spike type. Comprehensive re-sequencing Vrs1 revealed three two-rowed alleles (Vrs1.b2; Vrs1.b3; Vrs1.t1) and four six-rowed (vrs1.a1; vrs1.a2; vrs1.a3; vrs1.a4) in the natural population. However, the current knowledge about Vrs1 alleles and its distribution among Spanish barley subpopulations is still underexploited. We analyzed the gene in a panel of 215 genotypes, made of Spanish landraces and European cultivars. Among 143 six-rowed accessions, 57 had the vrs1.a1 allele, 83 were vrs1.a2, and three showed the vrs1.a3 allele. Vrs1.b3 was found in most two-rowed accessions, and a new allele was observed in 7 out of 50 two-rowed Spanish landraces. This allele, named Vrs1.b5, contains a ‘T’ insertion in exon 2, originally proposed as the causal mutation giving rise to the six-row vrs1.a2 allele, but has an additional upstream deletion that results in the change of 15 amino acids and a potentially functional protein. We conclude that eight Vrs1 alleles (Vrs1.b2, Vrs1.b3, Vrs1.b5, Vrs1.t1, vrs1.a1, vrs1.a2, vrs1.a3, vrs1.a4) discriminate two and six-rowed barleys. The markers described will be useful for DUS identification, plant breeders, and other crop scientists. |
|
| dc.description |
This work was supported by the Spanish Ministry of Economy, Industry and Competitiveness grants AGL2010-21929, AGL2013-48756-R, RFP2012-00015-00-00, RTA2012-00033-C03-02, and EUI2009-04075 (national code for Plant-KBBE project ExpResBar). CPC was funded by the Spanish Ministry of Economy, Industry and Competitiveness grant no. BES-2011-045905 (linked to project AGL2010-21929). TK and SS were supported by a research fund by the Ministry of Agriculture, Forestry, and Fisheries of Japan (Genomics for Agricultural Innovation grants no. TRS1002). SS was supported by a Grant-in-Aid from the Japan Society for the Promotion of Science (JSPS) Postdoctoral Fellow for Research Abroad and a Grant-in-Aid for Young Scientists (B) (no. 16 K18635). |
|
| dc.language |
eng |
|
| dc.publisher |
Springer Science+Business Media B.V., part of Springer Nature |
|
| dc.relation |
info:eu-repo/grantAgreement/MICINN//AGL2010-21929/ES/DESCUBRIMIENTO DE NUEVA VARIABILIDAD PARA LA MEJORA DE LA CEBADA EN ESPAÑA/ |
|
| dc.relation |
info:eu-repo/grantAgreement/MINECO//AGL2013-48756-R/ES/DESCUBRIMIENTO Y UTILIZACION DE LA VARIABILIDAD GENETICA QUE DETERMINA LA ADAPTACION DE LA CEBADA MEDIANTE HERRAMIENTAS GENETICAS, GENOMICAS/ |
|
| dc.relation |
info:eu-repo/grantAgreement/MINECO//RFP2012-00015-00-00/ES/ |
|
| dc.relation |
info:eu-repo/grantAgreement/MINECO//RTA2012-00033-C03-02/ES/Obtención de variedades de cebada de calidad con amplia adaptación en España/ |
|
| dc.relation |
info:eu-repo/grantAgreement/MICINN//EUI2009-04075/ES/EXPLOITING GENETIC VARIATION FOR RESISTANCE TO IMPORTANT PATHOGENS IN BARLEY/ |
|
| dc.relation |
info:eu-repo/grantAgreement/MICINN//BES-2011-045905/ES/BES-2011-045905/ |
|
| dc.relation |
Versió postprint del document publicat a: https://doi.org/10.1007/s11032-018-0816-z |
|
| dc.relation |
Molecular Breeding, 2018, vol. 38, p. 51 |
|
| dc.rights |
(c) Springer Science+Business Media B.V., part of Springer Nature, 2018 |
|
| dc.rights |
info:eu-repo/semantics/openAccess |
|
| dc.subject |
Barley |
|
| dc.subject |
Landraces |
|
| dc.subject |
Vrs1 |
|
| dc.subject |
SNP |
|
| dc.title |
Resequencing the Vrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike |
|
| dc.type |
info:eu-repo/semantics/article |
|
| dc.type |
info:eu-repo/semantics/acceptedVersion |
|