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Inactivation of rice starch branching enzyme IIb triggers broad and unexpected changes in metabolism by transcriptional reprogramming

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dc.creator Baysal, Can
dc.creator He, Wenshu
dc.creator Drapal, Margit
dc.creator Villorbina Noguera, Gemma
dc.creator Medina Piles, Vicente
dc.creator Capell Capell, Teresa
dc.creator Khush, Gurdev S.
dc.creator Zhu, Changfu
dc.creator Fraser, Paul
dc.creator Christou, Paul
dc.date 2020-10-05
dc.date.accessioned 2025-11-03T12:17:24Z
dc.date.available 2025-11-03T12:17:24Z
dc.identifier https://doi.org/10.1073/pnas.2014860117
dc.identifier 0027-8424
dc.identifier http://hdl.handle.net/10459.1/69729
dc.identifier.uri http://fima-docencia.ub.edu:8080/xmlui/handle/123456789/24450
dc.description Starch properties can be modified by mutating genes responsible for the synthesis of amylose and amylopectin in the endosperm. However, little is known about the effects of such targeted modifications on the overall starch biosynthesis pathway and broader metabolism. Here we investigated the effects of mutating the OsSBEIIb gene encoding starch branching enzyme IIb, which is required for amylopectin synthesis in the endosperm. As anticipated, homozygous mutant plants, in which OsSBEIIb was completely inactivated by abolishing the catalytic center and C-terminal regulatory domain, produced opaque seeds with depleted starch reserves. Amylose content in the mutant increased from 19.6 to 27.4% and resistant starch (RS) content increased from 0.2 to 17.2%. Many genes encoding isoforms of AGPase, soluble starch synthase, and other starch branching enzymes were up-regulated, either in their native tissues or in an ectopic manner, whereas genes encoding granule-bound starch synthase, debranching enzymes, pullulanase, and starch phosphorylases were largely down-regulated. There was a general increase in the accumulation of sugars, fatty acids, amino acids, and phytosterols in the mutant endosperm, suggesting that intermediates in the starch biosynthesis pathway increased flux through spillover pathways causing a profound impact on the accumulation of multiple primary and secondary metabolites. Our results provide insights into the broader implications of perturbing starch metabolism in rice endosperm and its impact on the whole plant, which will make it easier to predict the effect of metabolic engineering in cereals for nutritional improvement or the production of valuable metabolites.
dc.description We would like to acknowledge funding from Ministry of Economy and Competitiveness, Spain (RTI2018-097613-BI00 to C.Z., PGC2018-097655-B-I00 to P.C., and AGL2017-85377-R to T.C.); Generalitat de Catalunya Grant 2017 SGR 828 to the Agricultural Biotechnology and Bioeconomy Unit; and the European Union Framework Program DISCO (from discovery to final products: a next-generation pipeline for the sustainable generation of high-value plant products; Project 613513) to P.D.F.
dc.format application/pdf
dc.language eng
dc.publisher National Academy of Sciences
dc.relation info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097613-B-I00/ES/ELUCIDACION DEL MECANISMO RESPONSABLE DE LA ACUMULACION Y ESTABILIDAD DEL B-CAROTENO EN EL ENDOSPERMO DEL MAIZ/
dc.relation info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-097655-B-I00/ES/INVESTIGAR EL MECANISMO DE REACTIVACION DE PROMOTORES QUE SE ENCUENTRAN LATENTES EN EL ENDOSPERMO PARA SU USO EN INGENIERIA METABOLICA RESULTANDO EN PLANTAS NO TRANSGENICAS/
dc.relation info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-85377-R/ES/IMPACTO DE LAS PRACTICAS DE CULTIVO EN PLANTAS DE ARROZ QUE ACUMULAN UNA COMBINACION DE MICROBICIDAS EN EL ENDOSPERMO DE SUS SEMILLAS/
dc.relation Versió postprint del document publicat a: https://doi.org/10.1073/pnas.2014860117
dc.relation Proceedings of the National Academy of Sciences of the United States of America, 2020, vol. 117, num. 42, p. 26503-26512
dc.relation info:eu-repo/grantAgreement/EC/FP7/613513
dc.rights (c) Baysal, Can et al., 2020
dc.rights info:eu-repo/semantics/openAccess
dc.subject Endosperm
dc.subject High-amylose rice
dc.subject metabolomics
dc.subject Starch biosynthesis
dc.subject transcriptomics
dc.title Inactivation of rice starch branching enzyme IIb triggers broad and unexpected changes in metabolism by transcriptional reprogramming
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/acceptedVersion


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