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Novel processing technologies as compared to thermal treatment on the bioaccessibility and caco‑2 cell uptake of carotenoids from tomato and kale-based juices

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dc.creator Zhong, Siqiong
dc.creator Vendrell Pacheco, Mariona
dc.creator Heskitt, Brian
dc.creator Chitchumroonchokchai, Chureeporn
dc.creator Failla, Mark
dc.creator Sastry, Sudhir K.
dc.creator Francis, David M.
dc.creator Martín Belloso, Olga
dc.creator Elez Martínez, Pedro
dc.creator Kopec, Rachel E.
dc.date 2019
dc.date.accessioned 2025-11-03T12:17:14Z
dc.date.available 2025-11-03T12:17:14Z
dc.identifier https://doi.org/10.1021/acs.jafc.9b03666
dc.identifier 0021-8561
dc.identifier http://hdl.handle.net/10459.1/69723
dc.identifier.uri http://fima-docencia.ub.edu:8080/xmlui/handle/123456789/24320
dc.description This research aimed to measure the impact of novel food processing techniques, i.e., pulsed electric field (PEF) and ohmic heating (OH), on carotenoid bioaccessibility and Caco-2 cell uptake from tomato juice and high-pressure processing (HPP) and PEF on the same attributes from kale-based juices, as compared with raw (nonprocessed) and conventional thermally treated (TT) juices. Lycopene, β-carotene, and lutein were quantitated in juices and the micelle fraction using high-performance liquid chromatography (HPLC)-diode array detection and in Caco-2 cells using HPLC-tandem mass spectrometry. Tomato juice results were as follows: PEF increased lycopene bioaccessibility (1.5 ± 0.39%) by 150% (P = 0.01) but reduced β-carotene bioaccessibility (28 ± 6.2%) by 44% (P = 0.02), relative to raw juice. All processing methods increased lutein uptake. Kale-based juice results were as follows: TT and PEF degraded β-carotene and lutein in the juice. No difference in bioaccessibility or cell uptake was observed. Total delivery, i.e., the summation of bioaccessibility and cell uptake, of lycopene, β-carotene, and lutein was independent of type of processing. Taken together, PEF and OH enhanced total lycopene and lutein delivery from tomato juice to Caco-2 cells as well as TT, and may produce a more desirable product due to other factors (i.e., conservation of heat-labile micronutrients, fresher organoleptic profile). HPP best conserved the carotenoid content and color of kale-based juice and merits further consideration.
dc.description This work was supported by The Center for Advanced Processing and Packaging Studies (CAPPS), an NSF IUCRC Founded Center.
dc.format application/pdf
dc.language eng
dc.publisher American Chemical Society
dc.relation Versió postprint del document publicat a: https://doi.org/10.1021/acs.jafc.9b03666
dc.relation Journal of Agricultural and Food Chemistry, 2019, vol. 67, num. 36, p. 10185-10194
dc.rights (c) American Chemical Society, 2019
dc.rights info:eu-repo/semantics/openAccess
dc.subject Pulsed electric field
dc.subject Ohmic heating
dc.subject High-pressure processing
dc.subject Lycopene
dc.subject B-Carotene
dc.title Novel processing technologies as compared to thermal treatment on the bioaccessibility and caco‑2 cell uptake of carotenoids from tomato and kale-based juices
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/acceptedVersion


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