| dc.creator |
Granado-Serrano, Ana Belén |
|
| dc.creator |
Martín, María Angeles |
|
| dc.creator |
Haegeman, Guy |
|
| dc.creator |
Goya, Luis |
|
| dc.creator |
Bravo, Laura |
|
| dc.creator |
Ramos, Sonia |
|
| dc.date |
2010 |
|
| dc.date.accessioned |
2025-11-03T12:15:30Z |
|
| dc.date.available |
2025-11-03T12:15:30Z |
|
| dc.identifier |
https://doi.org/10.1017/S0007114509991747 |
|
| dc.identifier |
0007-1145 |
|
| dc.identifier |
http://hdl.handle.net/10459.1/64884 |
|
| dc.identifier.uri |
http://fima-docencia.ub.edu:8080/xmlui/handle/123456789/24121 |
|
| dc.description |
The dietary flavonoid epicatechin has been reported to exhibit a wide range of biological activities. The objective of the present study was to
investigate the time-dependent regulation by epicatechin on the activity of the main transcription factors (NF-kB, activator protein-1 (AP-1)
and nuclear transcription factor erythroid 2p45-related factor (Nrf2)) related to antioxidant defence and survival and proliferation pathways in
HepG2 cells. Treatment of cells with 10mM-epicatechin induced the NF-kB pathway in a time-dependent manner characterised by increased
levels of IkB kinase (IKK) and phosphorylated inhibitor of kB subunit-a (p-IkBa) and proteolytic degradation of IkB, which was
consistent with an up-regulation of the NF-kB-binding activity. Time-dependent activation of the AP-1 pathway, in concert with enhanced
c-Jun nuclear levels and induction of Nrf2 translocation and phosphorylation were also demonstrated. Additionally, epicatechin-induced NF-kB
and Nrf2 were connected to reactive oxygen species intracellular levels and to the activation of cell survival and proliferation pathways, being
phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) and extracellular regulated kinase (ERK) associated to Nrf2 modulation and ERK
to NF-kB induction. These data suggest that the epicatechin-induced survival effect occurs by the induction of redox-sensitive transcription factors
through a tight regulation of survival and proliferation pathways. |
|
| dc.description |
The present study was supported by the grants AGL2004-302, AGL2007-64042 and CSD2007-00063 from the Spanish Ministry of Science and Innovation (CICYT) and 200870 I198 (CSIC). A. B. G.-S. is a predoctoral fellow of the Spanish Ministry of Science and Education. |
|
| dc.language |
eng |
|
| dc.publisher |
Cambridge University Press |
|
| dc.relation |
info:eu-repo/grantAgreement/MEC//AGL2004-302/ES/ |
|
| dc.relation |
info:eu-repo/grantAgreement/MEC//AGL2007-64042/ES/EVALUACION IN VITRO E IN VIVO DEL POTENCIAL QUIMIOPREVENTIVO DE FLAVANOLES DE LA DIETA FRENTE AL DESARROLLO DE CANCER COLONICO/ |
|
| dc.relation |
info:eu-repo/grantAgreement/MEC//CSD2007-00063/ES/Nuevos Ingredientes de Alimentos Funcionales para Mejorar la Salud/ |
|
| dc.relation |
Reproducció del document publicat a https://doi.org/10.1017/S0007114509991747 |
|
| dc.relation |
British Journal of Nutrition, 2010, vol. 103, p. 168–179 |
|
| dc.rights |
(c) Cambridge University Press, 2010 |
|
| dc.rights |
info:eu-repo/semantics/openAccess |
|
| dc.subject |
Epicatechin |
|
| dc.subject |
NF-kB signalling |
|
| dc.subject |
Activator protein-1 |
|
| dc.subject |
Nuclear factor-erythroid 2p45-related factor-2 |
|
| dc.title |
Epicatechin induces NF-kB, activator protein-1 (AP-1) and nuclear transcription factor erythroid 2p45-related factor-2 (Nrf2) via phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) and extracellular regulated kinase (ERK) signalling in HepG2 cells |
|
| dc.type |
info:eu-repo/semantics/article |
|
| dc.type |
info:eu-repo/semantics/publishedVersion |
|