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| dc.creator | Tailor, A. | |
| dc.creator | Tomlinson, Annette, 1942- | |
| dc.creator | Salas Martínez, Azucena | |
| dc.creator | Panés Díaz, Julià | |
| dc.creator | Granger, D. Neil | |
| dc.creator | Flower, Roderick J. | |
| dc.creator | Perretti, M. | |
| dc.date | 2011-07-07T12:30:40Z | |
| dc.date | 2011-07-07T12:30:40Z | |
| dc.date | 1999 | |
| dc.date.accessioned | 2024-12-16T10:27:15Z | |
| dc.date.available | 2024-12-16T10:27:15Z | |
| dc.identifier | 0017-5749 | |
| dc.identifier | http://hdl.handle.net/2445/18663 | |
| dc.identifier | 551417 | |
| dc.identifier | 10517906 | |
| dc.identifier.uri | http://fima-docencia.ub.edu:8080/xmlui/handle/123456789/22149 | |
| dc.description | BACKGROUND A previous study showed that the glucocorticoid dexamethasone, at doses of 100 ¿g/kg and above, inhibited leucocyte adhesion to rat mesenteric postcapillary venules activated with interleukin 1ß (IL-1ß), as assessed by videomicroscopy. AIMS To identify whether the adhesion molecule, intercellular adhesion molecule 1 (ICAM-1), or the chemokine KC could be targeted by the steroid to mediate its antiadhesive effect. METHODS Rat mesenteries were treated with IL-1ß (20 ng intraperitoneally) and the extent of leucocyte adhesion measured at two and four hours using intravital microscopy. Rats were treated with dexamethasone, and passively immunised against ICAM-1 or KC. Endogenous expression of these two mediators was validated by immunohistochemistry, ELISA, and the injection of specific radiolabelled antibodies. RESULTS Dexamethasone greatly reduced IL-1ß induced leucocyte adhesion, endothelial expression of ICAM-1 in the postcapillary venule, and release of the mast cell derived chemokine KC. Injection of specific antibodies to the latter mediators was also extremely effective in downregulating (>80%) IL-1ß induced leucocyte adhesion. CONCLUSIONS Induction by IL-1ß of endogenous ICAM-1 and KC contributes to leucocyte adhesion to inflamed mesenteric vessels. Without excluding other possible mediators, these data clearly show that dexamethasone interferes with ICAM-1 expression and KC release from mast cells, resulting in suppression of leucocyte accumulation in the bowel wall, which is a prominent feature of several gastrointestinal pathologies. | |
| dc.format | 8 p. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | BMJ Group | |
| dc.relation | Reproducció digital del document publicat a: http://dx.doi.org/10.1136/gut.45.5.705 | |
| dc.relation | Gut, 1999, vol. 45, núm. 5, p. 705-712 | |
| dc.relation | http://dx.doi.org/10.1136/gut.45.5.705 | |
| dc.rights | (c) BMJ Publishing Group Ltd and British Society of Gastroenterology, 1999 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Articles publicats en revistes (Medicina) | |
| dc.subject | Biologia molecular | |
| dc.subject | Leucòcits | |
| dc.subject | Molecular biology | |
| dc.subject | Leukocytes | |
| dc.title | Dexamethasone inhibition of leucocyte adhesion to rat mesenteric postcapillary venules: role of intercellular adhesion molecule 1 and KC | |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:eu-repo/semantics/publishedVersion |
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