| dc.creator |
Tailor, A. |
|
| dc.creator |
Tomlinson, Annette, 1942- |
|
| dc.creator |
Salas Martínez, Azucena |
|
| dc.creator |
Panés Díaz, Julià |
|
| dc.creator |
Granger, D. Neil |
|
| dc.creator |
Flower, Roderick J. |
|
| dc.creator |
Perretti, M. |
|
| dc.date |
2011-07-07T12:30:40Z |
|
| dc.date |
2011-07-07T12:30:40Z |
|
| dc.date |
1999 |
|
| dc.date.accessioned |
2024-12-16T10:27:15Z |
|
| dc.date.available |
2024-12-16T10:27:15Z |
|
| dc.identifier |
0017-5749 |
|
| dc.identifier |
http://hdl.handle.net/2445/18663 |
|
| dc.identifier |
551417 |
|
| dc.identifier |
10517906 |
|
| dc.identifier.uri |
http://fima-docencia.ub.edu:8080/xmlui/handle/123456789/22149 |
|
| dc.description |
BACKGROUND A previous study showed that the glucocorticoid dexamethasone, at doses of 100 ¿g/kg and above, inhibited leucocyte adhesion to rat mesenteric postcapillary venules activated with interleukin 1ß (IL-1ß), as assessed by videomicroscopy. AIMS To identify whether the adhesion molecule, intercellular adhesion molecule 1 (ICAM-1), or the chemokine KC could be targeted by the steroid to mediate its antiadhesive effect. METHODS Rat mesenteries were treated with IL-1ß (20 ng intraperitoneally) and the extent of leucocyte adhesion measured at two and four hours using intravital microscopy. Rats were treated with dexamethasone, and passively immunised against ICAM-1 or KC. Endogenous expression of these two mediators was validated by immunohistochemistry, ELISA, and the injection of specific radiolabelled antibodies. RESULTS Dexamethasone greatly reduced IL-1ß induced leucocyte adhesion, endothelial expression of ICAM-1 in the postcapillary venule, and release of the mast cell derived chemokine KC. Injection of specific antibodies to the latter mediators was also extremely effective in downregulating (>80%) IL-1ß induced leucocyte adhesion. CONCLUSIONS Induction by IL-1ß of endogenous ICAM-1 and KC contributes to leucocyte adhesion to inflamed mesenteric vessels. Without excluding other possible mediators, these data clearly show that dexamethasone interferes with ICAM-1 expression and KC release from mast cells, resulting in suppression of leucocyte accumulation in the bowel wall, which is a prominent feature of several gastrointestinal pathologies. |
|
| dc.format |
8 p. |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
BMJ Group |
|
| dc.relation |
Reproducció digital del document publicat a: http://dx.doi.org/10.1136/gut.45.5.705 |
|
| dc.relation |
Gut, 1999, vol. 45, núm. 5, p. 705-712 |
|
| dc.relation |
http://dx.doi.org/10.1136/gut.45.5.705 |
|
| dc.rights |
(c) BMJ Publishing Group Ltd and British Society of Gastroenterology, 1999 |
|
| dc.rights |
info:eu-repo/semantics/openAccess |
|
| dc.source |
Articles publicats en revistes (Medicina) |
|
| dc.subject |
Biologia molecular |
|
| dc.subject |
Leucòcits |
|
| dc.subject |
Molecular biology |
|
| dc.subject |
Leukocytes |
|
| dc.title |
Dexamethasone inhibition of leucocyte adhesion to rat mesenteric postcapillary venules: role of intercellular adhesion molecule 1 and KC |
|
| dc.type |
info:eu-repo/semantics/article |
|
| dc.type |
info:eu-repo/semantics/publishedVersion |
|