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Fluorescence imaging applied to the study of plant tissues

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dc.contributor Juan Capdevila, Anna de
dc.contributor Marro, Mónica
dc.creator Gómez Sánchez, Adrián
dc.date 2018-02-21T13:26:40Z
dc.date 2018-02-21T13:26:40Z
dc.date 2018-01
dc.date.accessioned 2024-12-16T10:26:14Z
dc.date.available 2024-12-16T10:26:14Z
dc.identifier http://hdl.handle.net/2445/120071
dc.identifier.uri http://fima-docencia.ub.edu:8080/xmlui/handle/123456789/20736
dc.description Treballs Finals de Grau de Química, Facultat de Química, Universitat de Barcelona, Any: 2018, Tutores: Anna de Juan Capdevila, Mónica Marro Sánchez
dc.description Plant tissues have natural fluorophores on their structure. When plants are exposed to an environmental stress, plant morphology, structural elements and content of fluorophores may vary. Three population of Oryza Sativa (rice) samples grew under control and stress conditions (watered with cadmium 50 μM and 1000 μM solutions). Fluorescence imaging allowed monitoring natural fluorophores such as chlorophylls or lignins in leave tissues. The information from fluorescence imaging was interpreted by the chemometric tool multivariate curve resolution alternating least squares (MCR – ALS) method which provides the pure fluorescence signatures and distribution maps of the fluorophores presents in the plants analyzed. Plants exposed to cadmium were more yellow indicating a lower concentration of chlorophylls. In leaves cross-sections, biological structures seem to be weaker than in non-contaminated plants. The leaf length also decreased in Cd-exposed populations. The fluorescence signatures and distribution maps of three types of chlorophyll and two lignin compounds were resolved. The use of fluorescence imaging techniques combined with chemometric tools are useful to obtain relevant biological, morphological and chemical information about compounds presents in plant tissues.
dc.format 55 p.
dc.format application/pdf
dc.language eng
dc.rights cc-by-nc-nd (c) Gómez, 2018
dc.rights http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights info:eu-repo/semantics/openAccess
dc.source Treballs Finals de Grau (TFG) - Química
dc.subject Quimiometria
dc.subject Cèl·lules i teixits vegetals
dc.subject Metalls pesants
dc.subject Treballs de fi de grau
dc.subject Chemometrics
dc.subject Plant cells and tissues
dc.subject Heavy metals
dc.subject Bachelor's theses
dc.title Fluorescence imaging applied to the study of plant tissues
dc.title Imagen hiperespectral de fluorescencia aplicada al estudio de tejidos vegetales
dc.type info:eu-repo/semantics/bachelorThesis


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