| dc.creator |
Carreras, Alba |
|
| dc.creator |
Rojas, Mauricio |
|
| dc.creator |
Tsapikouni, Theodora |
|
| dc.creator |
Montserrat Canal, José Ma. |
|
| dc.creator |
Navajas Navarro, Daniel |
|
| dc.creator |
Farré Ventura, Ramon |
|
| dc.date |
2010-09-10T10:55:12Z |
|
| dc.date |
2010-09-10T10:55:12Z |
|
| dc.date |
2010-07-06 |
|
| dc.date.accessioned |
2024-12-16T10:25:12Z |
|
| dc.date.available |
2024-12-16T10:25:12Z |
|
| dc.identifier |
1465-9921 |
|
| dc.identifier |
http://hdl.handle.net/2445/13645 |
|
| dc.identifier |
584176 |
|
| dc.identifier |
20604943 |
|
| dc.identifier.uri |
http://fima-docencia.ub.edu:8080/xmlui/handle/123456789/19007 |
|
| dc.description |
Background: The aim was to test the hypothesis that the blood serum of rats subjected to recurrent airway obstructions mimicking obstructive sleep apnea (OSA) induces early activation of bone marrow-derived mesenchymal stem cells (MSC) and enhancement of endothelial wound healing. Methods: We studied 30 control rats and 30 rats subjected to recurrent obstructive apneas (60 per hour, lasting 15 s each, for 5 h). The migration induced in MSC by apneic serum was measured by transwell assays. MSC-endothelial adhesion induced by apneic serum was assessed by incubating fluorescent-labelled MSC on monolayers of cultured endothelial cells from rat aorta. A wound healing assay was used to investigate the effect of apneic serum on endothelial repair. Results: Apneic serum showed significant increase in chemotaxis in MSC when compared with control serum: the normalized chemotaxis indices were 2.20 +- 0.58 (m +- SE) and 1.00 +- 0.26, respectively (p < 0.05). MSC adhesion to endothelial cells was greater (1.75 +- 0.14 -fold; p < 0.01) in apneic serum than in control serum. When compared with control serum, apneic serum significantly increased endothelial wound healing (2.01 +- 0.24 -fold; p < 0.05). Conclusions: The early increases induced by recurrent obstructive apneas in MSC migration, adhesion and endothelial repair suggest that these mechanisms play a role in the physiological response to the challenges associated to OSA. |
|
| dc.format |
7 p. |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
BioMed Central |
|
| dc.relation |
Reproducció del document publicat a http://dx.doi.org/10.1186/1465-9921-11-91 |
|
| dc.relation |
Respiratory Research 2010, 11:91, p. 1-7 |
|
| dc.relation |
http://dx.doi.org/10.1186/1465-9921-11-91 |
|
| dc.rights |
cc-by (c) Carreras et al., 2010 |
|
| dc.rights |
http://creativecommons.org/licenses/by/2.0 |
|
| dc.rights |
info:eu-repo/semantics/openAccess |
|
| dc.source |
Articles publicats en revistes (Ciències Fisiològiques) |
|
| dc.subject |
Trastorns respiratoris |
|
| dc.subject |
Fisiologia animal |
|
| dc.subject |
Respiration disorders |
|
| dc.subject |
Physiology, Comparative |
|
| dc.title |
Obstructive apneas induce early activation of mesenchymal stem cells and enhancement of endothelial wound healing |
|
| dc.type |
info:eu-repo/semantics/article |
|
| dc.type |
info:eu-repo/semantics/publishedVersion |
|