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Crystallization and diffraction of single-crystals at ALBA Synchrotron to structure determination of a Relaxase protein

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dc.contributor Sainz García, Daniel
dc.contributor Boer, Roeland
dc.creator Guevara Puig, Tibisay
dc.date 2016-03-03T15:38:40Z
dc.date 2016-03-03T15:38:40Z
dc.date 2016-01-11
dc.date.accessioned 2024-12-16T10:22:04Z
dc.date.available 2024-12-16T10:22:04Z
dc.identifier http://hdl.handle.net/2445/96106
dc.identifier.uri http://fima-docencia.ub.edu:8080/xmlui/handle/123456789/13702
dc.description Treballs Finals de Grau de Química, Facultat de Química, Universitat de Barcelona, Any: 2016, Tutor: Daniel Sainz García i Roeland Boer
dc.description The aim of the present work is the structure description of two variant (full length and Nterminal) of a protein with Relaxase activity. Both pure variants were crystallized using sitting drop vapor diffusion technique. Crystals obtained were optimized increasing its quality and some of them were treated to derivatize it with metals. Diffraction of crystals and pre-treatment of protein were integrally performed in ALBA Synchrotron (Barcelona) installations. In BL-13 XALOC beamline were collected the data which were processed and evaluated as good quality data. The phase problem solution was not possible by applying Molecular Replacement nor detecting the anomalous signal of derivatives crystals. The lack of phases did not permit to solve the electron density equation, and therefore, it was not possible to complete the model building
dc.format 59 p.
dc.format application/pdf
dc.language eng
dc.rights cc-by-nc-nd (c) Guevara, 2016
dc.rights http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights info:eu-repo/semantics/openAccess
dc.source Treballs Finals de Grau (TFG) - Química
dc.subject Sincrotrons
dc.subject Cristal·lització
dc.subject Proteïnes
dc.subject Treballs de fi de grau
dc.subject Synchrotrons
dc.subject Crystallization
dc.subject Proteins
dc.subject Bachelor's theses
dc.title Crystallization and diffraction of single-crystals at ALBA Synchrotron to structure determination of a Relaxase protein
dc.title Cristal•lització i difracció de monocristalls al Sincrotró ALBA per a la determinació estructural d’una proteïna Relaxassa
dc.type info:eu-repo/semantics/bachelorThesis


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